GL Sciences' MonoTip TiO2 Titanium Dioxide Phosphorylated Peptide Enrichment Pipette Tips

A completely new base material for low pressure, pipette-tip enrichment of phosphorylated peptides has been developed by GL Sciences. This unique material consists of Titanium Dioxide coated on the surface of a porous silica monolithic disc, which is immobilized in a pipette tip. Tips are available in standard 10-200uL size in packages of 8 or 96. Sample capacity on the 10-200uL tips is approximately 15 ug for tyrosine phosphopeptide, and an average of about 5 ug total phosphopeptide binding of real-world samples.

MonoTip TiO2 offers greater selectivity for phosphopeptides than alternative methods, such as IMAC (immobilized metal affinity chromatography). Typically, IMAC shows significant binding of non-phosphorylated peptides which have multiple acidic residues. This problem is often eliminated by using MonoTip TiO2.

A list of references is shown below which detail the benefits of MonoTip and Titansphere (HPLC version of MonoTip). Please email GL Sciences for particular papers as we are able to provide some of these papers in a PDF format.

Click here for a copy of the Care and Use Manual in PDF format

Click here to find an authorized Dealer in your Country

USA Order No.	MonoTip TiO Ordering Information	USA Price	Unit	GL Sciences Japan Part No.
1400008	MonoTip TiO, Pipette Tip, 10 - 200uL, 8/pkg	90.00	8/pkg	5010-21006
1400096	MonoTip TiO, Pipette Tip, 10 - 200uL, 96/pkg	390.00	96/pkg	5010-21005

Additional References shown below. A listing of PDF documents from ASMS (American Society of Mass Spectrometry) relating to TiO2 use for phosphopeptide enrichment can be found here.

Phosphopeptide extraction

Online, For digest of purified protein
● A. Schlosser, J. T. Vanselow, and A. Kramer, “ Mapping of phosphorylation sites by a multi-protease approach with specific phosphopeptide enrichment and NanoLC-MS/MS analysis ”, Analytical Chemistry 2005 Aug 15;77(16):5243-50.

● M. W. H. Pinkse, P. M. Uitto, M. J. Hilhorst, B. Ooms, and A. J. R. Heck, “ Selective isolation at the femtomole level of phosphopeptides from proteolytic digests using 2D-NanoLC-ESI-MS/MS and titanium oxide precolumns ”, Analytical Chemistry 2004 Jul 15;76(14):3935-43.

● I. Kuroda, Y. Shintani, M. Motokawa, S. Abe, and M, Furuno, “ Phosphopeptide-selective Column-switching RP-HPLC with a Titania Precolumn ”, Analytical Sciences 2004 Sep;20(9):1313-9.
PDF Available Here

●I. Kuroda, M. Motokawa, T. Fukaya, and M, Takahashi, “ Selective Detection of Phosphopeptides by Using 2-dimentional Capillary HPLC/MS ”, Poster made in 2004 for external release by GL Sciences inc. PDF. file is available.

Offline, For digest of protein mixture
● M. R. Larsen, T. E. Thingholm, O. N. Jensen, P. Roepstorff, and T. J. D. Jorgensen, “ Highly selective enrichment of phosphorylated peptides from peptide mixtures using titanium dioxide microcolumns ”, Molecular & Cellular Proteomics 2005; 4: 873-886.

● Yasuhiro Hirano, Masashi Segawa, Fumiko S. Ouchi¶, Yoshio Yamakawa¶, Kazuhiro Furukawa, Kunio Takeyasu, and Tsuneyoshi Horigome1
Dissociation of Emerin from Barrier-to-autointegration Factor Is Regulated through Mitotic Phosphorylation of Emerin in a Xenopus Egg Cell-free System^*
J. Biol. Chem., Vol. 280, Issue 48, 39925-39933, December 2, 2005

Phosphosugar extraction

Online, For phosphosugars in extract of plant

● Y. Sekiguchi, N. Mitsuhashi, Y. Inoue, H. Yagisawa, and T. Mimura, “ Analysis of sugar phosphates in plants by ion chromatography on a titanium dioxide column with pulsed amperometric detection ”, Journal of Chromatography A 2004 Jun 11;1039(1-2):71-6.

● Y. Sekiguchi, N. Mitsuhashi, and T. Mimura, “ Sugar Phosphates Changes in Arabidopsis in Response to Phosphate Nutrition Measured by Improved Ion Chromatography with Pulsed Amperometric Detection Combined with a Titanium Dioxide Column ”, Plant Biotechnology 2004 21(2):143-150.

Glyphosate extraction

● T. Ishiwata, K. Takai, H. Okada and K. Ohashi, “ ANALYSIS OF PHOSPHORUS-CONTAINING AMINO ACID-TYPE HERBICIDES AND THEIR METABOLITES IN HUMAN BLOOD BY EXTRACTION WITH A TITANSPHERE TiO COLUMN AND CAPILLARY ELECTROPHORESIS ”, Jpn J Forensic Toxicol, Vol 22, No.1, 2004:7-12.

New assay method for ATP-dependant protein

● Y. Kimura, S. Shibasaki, K. Morisato, N. Ishizuka, H. Minakuchi, K. Nakanishi, M. Matsuo, T. Amachi, M. Ueda, and K. Ueda, “ Microanalysis for MDR1 ATPase by high-performance liquid chromatography with a titanium dioxide column ”, Analytical Biochemistry 2004 Mar 15;326(2):262-6.