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 Rapidly perform tryptic digests at room temperature |
 Immobilized Trypsin (TPCK) does not digest itself as occurs in solution |
 Monolith disk contained in Pipette Tips provide better flow properties than packed-bed pipette tips |
 click here for ordering information | ||||
A completely new base material for low pressure, pipette-tip tryptic digestion of proteins has been developed by GL Sciences. This unique material consists of Trypsin (TPCK) immobilized on the surface of a porous silica monolithic disc, which is embedded in a pipette tip. Tips are available in 10-200uL sizes in packages of 8 or 96.  Figure 1 The chromatogram above shows an HPLC analysis of denatured transferrin before and after digestion with MonoTip Trypsin. The 0.1mg/ml solution of transferrin was cycled approximately 10 times through a MonoTip Trypsin pipette tip at room temperature. For additional information and details about MonoTip Trypsin, click here. Figure 2 The chromatograms below compare the results of tryptic digest in under 15 minutes using MonoTip Trypsin at room temperature versus traditional bulk digestion for 15 hours at 37°C. The top chromatogram shows the results from the MonoTip digest and the lower chromatogram shows the results from the traditional bulk, in-solution digest. As indicated, the results show MonoTip Trypsin does a very efficient digestion in a fraction of the time of traditional methods. (click on image below to enlarge)  | ||||
| USA Order No. | MonoTip Trypsin Ordering Information | USA List | Unit | Japan Part No. |
| 1405008 | MonoTip Trypsin, Pipette Tip, 10 - 200uL, 8/pkg | 59.00 | 8/pkg | 5010-21011 |
| 1405024 | MonoTip Trypsin, Pipette Tip, 10 - 200uL, 24/pkg | 139.00 | 24/pkg | 5020-21012 |
| 1405096 | MonoTip Trypsin, Pipette Tip, 10 - 200uL, 96/pkg | 479.00 | 96/pkg | 5010-21010 |
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