| Key Benefits and Features of Inertsil Peptide 100A Column - Part of the Advanced Inertsil Series of Phases |
 Higher surface area than 300A provides better separation capabilities for smaller peptides |
 Rigid silica formulation provides physical and chemical stability |
 Exceptional reproducibility |
 Low operating pressures even in highly aqueous conditions |
| The new Inertsil Peptide column compliments the WP300 Series of Wide-Pore columns for protein and large peptide analysis. Whereas proteins and large peptides benefit from the increase surface area provided by 300A pores, smaller peptides, for example from 5 - 20 amino acid residues. The WP300 series currently consists of WP300C8, WP300C18, WP300Diol, WP300C4, WP300SIL and Peptide C18. | Inertsil Peptide columns are available in 5 micron particle size in column configurations ranging from capillary to 50mm ID preparative sizes. | |||
Figure 1 - Peptide Separation on Inertsil Peptide (C18) Column | ||||
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| Particle Size(s) | Particle Shape | Surface Area | Pore Size | Pore Volume | Silica Purity | Bonded Phase | End- Capping | Carbon Load | pH Range* |
| 5 µm | Spherical | 450 m2/g | 100Å | 1.05 mL/g | 99.999 | Peptide C18 | Yes | 15% | 2 - 9 |
| * Inertsil phases are known to provide excellent results and long column life at pH levels of 9 or 10. However, optimum column life will likely be achieved by operating at a pH of between 2 and 7.5. | |||||||||
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