You don’t have to change very much about your standard, run-of-the-mill gradient HPLC systems to achieve sub-minute separations with peaks widths under 1.0 seconds. In fact, all you really need is GL Sciences' UltraFast Kit, which consists of:
 
   Inertsil high-speed 3 micron 50 x 4.6 or 50 x 2.1mm columns
   Caloratherm eluent heater from Selerity Technologies, Inc.
   Optimized tubing and fittings to minimize dead volume
   Detailed instructions & discussion of the critical parameters that make UltraFast separations possible.

Left - FAST Peptide Separation (Gly-Tyr, Val-Tyr-Val, angiotensin II, Leu enkephalin, Met enkephalin, in order of elution) chromatograms at velocities of 7 mm/s (5 mL/min) using an ordinary HPLC (top) with 4.6 x 50 mm column and 14 mm/s  (2 mL/min) using a UPLC^TM with 2.1 x 50 mm column (bottom).  In both cases, a 5-35% ACN linear gradient with 0.2% HOAc buffer and Inertsil^TM ODS3 – 3 µm stationary phase were used.
Below - FAST TCA Separation

In other words, you don’t need to go out and spend a fortune on one of the new, ultra high-pressure systems that are currently in vogue to meet the increases in productivity demanded by today’s results oriented environment.  And even if your budget allows you to upgrade to a new, ultra high pressure system, better control of the operational parameters using GL Sciences’ UltraFast™ Kit can result in further dramatic improvements in chromatography and speed.

The fact is that the critical elements of a good chromatographic separation remain the stationary phase, the mobile phase, good mass transfer and minimized system dead volume.  GL Sciences’ UltraFast Kit provides all these critical elements to success.

For more information on what our products can do maximize productivity in your lab, contact GL Sciences by phone (310)265-4424  or email and request information on our UltraFast Kit.

GL Sciences’ UltraFast Kit provides detailed information on making almost any HPLC ultra fast, and particular attention is given to the following LC systems: Waters Ultra Performance LC™, Waters Acquity™ UPLC™, Waters Alliance™ configured with one or multiple columns, and Agilent 1100™ series configured as an open-access system.  Step-by-step instructions are included along with detailed photographs in each UltraFast Kit.  Additionally, GL Sciences’ can assist in converting just about any other system to provide much faster and better results than ever before possible. 

Currently, three UltraFast Kit configurations are "standard," and differ only in the hardware packages.  These three variations include "standard" hardware for use with 2.1 - 4.6mm id columns, "microbore" hardware for use with 1.0 - 2.1mm columns, and "open access" hardware for use with an open access system which will see a variety of column types and configurations.

    (site section still under construction as of 4/30/07.  More to come during the coming week!)

(Basic ordering information is shown below.  For additional details, click button at above which includes more information about the hardware kits included.  Every kit includes detailed setup information and suggestions for optimizing results.  Most customers select one each of Inertsil 3 micron ODS-3 and Inertsil 3 micron C8-3, but any Inertsil 3-Series phase can be ordered as desired.
 

UPLC® is a trademark of Waters Corporation.  Caloratherm is a trademark of Selerity Technologies, Inc.